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human cb1 receptor  (Revvity)


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    Revvity human cb1 receptor
    Human Cb1 Receptor, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cb1+receptor/10__1038_slash_s41591___023___02381___w-572-5-15?v=Revvity
    Average 91 stars, based on 10 article reviews
    human cb1 receptor - by Bioz Stars, 2026-08
    91/100 stars

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    Image Search Results


    Effects of CB1 knockdown on GAD67 expression in primary cultured neurons and METH-induced CPP. ( A ) Vehicle group was treated with vehicle (5% DMSO, 5% tween-20, 90% PBS) as the same amount of JWH-210 for 24 h. JWH-210 group was treated with 0.1 μM JWH-210 for 24 h. Mock + JWH-210 and CB1 overexpression (OE) + JWH-210 groups were treated with mock or CB1 ORF lentiviral particles (5 MOI) for 24 h before treatment with vehicle or 0.1 μM JWH-210 for 24 h. Immunostaining of CB1, Myc, and GAD67 was performed with specific antibodies (Scale bar: 200 μm). ( B ) Cultured neurons were treated with control or CB1 shRNA lentiviral particles. mRNA levels of ( C ) CB1 and ( D ) GAD67 were confirmed by using qPCR with each specific primer and normalized to the relative amplification of GAPDH. Data are expressed as the mean ± S.E. ( n = 6; Student’s t -test; * p < 0.05 and ** p < 0.01 vs. vehicle group). ( E ) After CB1 knockdown, 0.3 mg/kg METH-induced CPP scores (s) was measured by CPP test. Data are expressed as the mean ± S.E. ( n = 8) and were analyzed using two-way ANOVA followed by Bonferroni post-hoc t -test (* p < 0.05 and ** p < 0.01 vs. each saline group; # ## p < 0.001 vs. Control shRNA/METH group). OE: overexpression. TUJ1: beta-tubulin III. METH: methamphetamine.

    Journal: International Journal of Molecular Sciences

    Article Title: Cannabinoid Receptor Type 1 Regulates Drug Reward Behavior via Glutamate Decarboxylase 67 Transcription

    doi: 10.3390/ijms221910486

    Figure Lengend Snippet: Effects of CB1 knockdown on GAD67 expression in primary cultured neurons and METH-induced CPP. ( A ) Vehicle group was treated with vehicle (5% DMSO, 5% tween-20, 90% PBS) as the same amount of JWH-210 for 24 h. JWH-210 group was treated with 0.1 μM JWH-210 for 24 h. Mock + JWH-210 and CB1 overexpression (OE) + JWH-210 groups were treated with mock or CB1 ORF lentiviral particles (5 MOI) for 24 h before treatment with vehicle or 0.1 μM JWH-210 for 24 h. Immunostaining of CB1, Myc, and GAD67 was performed with specific antibodies (Scale bar: 200 μm). ( B ) Cultured neurons were treated with control or CB1 shRNA lentiviral particles. mRNA levels of ( C ) CB1 and ( D ) GAD67 were confirmed by using qPCR with each specific primer and normalized to the relative amplification of GAPDH. Data are expressed as the mean ± S.E. ( n = 6; Student’s t -test; * p < 0.05 and ** p < 0.01 vs. vehicle group). ( E ) After CB1 knockdown, 0.3 mg/kg METH-induced CPP scores (s) was measured by CPP test. Data are expressed as the mean ± S.E. ( n = 8) and were analyzed using two-way ANOVA followed by Bonferroni post-hoc t -test (* p < 0.05 and ** p < 0.01 vs. each saline group; # ## p < 0.001 vs. Control shRNA/METH group). OE: overexpression. TUJ1: beta-tubulin III. METH: methamphetamine.

    Article Snippet: CB1 human open reading frame (ORF) clone lentiviral particles (c-Myc tagged) were obtained from Origene (Rockville, MD, USA) for CB1 overexpression.

    Techniques: Expressing, Cell Culture, Over Expression, Immunostaining, shRNA, Amplification